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海洋异养硝化-好氧反硝化菌y6同步脱氮除碳特性_论文

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中国环境科学 2017,37(2):686~695 China Environmental Science 海洋异养硝化-好氧反硝化菌 y6 同步脱氮除碳特性 王骁静,于德爽,李 津*,都松东,周 同,吴国栋 (青岛大学环境科学与工程学院,山东 青岛 266071) 摘要: 从胶州湾海底沉积物中分离筛选出一株异养硝化-好氧反硝化菌株 y6,通过菌株 y6 的形态以及生理生化特性和 16S rRNA 基因序列 的分析,鉴定该菌株属于克雷伯氏菌属(Klebsiella sp.).在不同的环境条件下,测定菌株 y6 的生长情况和脱氮能力,研究其同步脱氮除碳特性. 实验结果表明,菌株 y6 的最佳碳源为柠檬酸三钠,最适宜 pH 值为 7.0,最适合的 C/N 为 17.菌株 y6 在以 NH4Cl、KNO3 和 NaNO2 为唯一氮 源的反应系统中均有较好的脱氮效果,去除率分别为 99.67%、100%、99.20%.菌株 y6 在脱氮的同时能高效地去除有机物,COD 的去除率分 别为 82.17%、95.75%和 97.83%.菌株 y6 在硝化过程中没有亚硝态氮和硝态氮的积累.在按不同比例混合氮源的反应系统内,首先进行的是 硝态氮的好氧反硝化,随后进行的是氨氮、亚硝态氮和 COD 的去除.在有亚硝态氮存在时氨氮的去除率略低,亚硝态氮会影响 y6 的异养硝 化过程,异养硝化对好氧反硝化过程没有影响. 关键词:异养硝化-好氧反硝化;海洋菌株;克雷伯氏菌 y6;同步脱氮除碳 中图分类号:X172 文献标识码:A 文章编号:1000-6923(2017)02-0686-10 Separation identification and the characteristics research of simultaneous removal of nitrogen and carbon about Marine heterotrophic nitrification and aerobic denitrification strain y6. WANG Xiao-jing, YU De-shuang, LI Jin*, DU Song-dong, ZHOU Tong, WU Guo-dong (School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China). China Environmental Science, 2017,37(2):686~695 Abstract:A heterotrophic nitrification-aerobic denitrification strain, named y6, was isolated from sediment of Jiaozhou Bay. It was identified as Klebsiella sp. based on the morphological, physiological and analysis of 16S rRNA gene sequence. Under different environmental conditions, measured strain y6’s growth situation and denitrification ability, so as to analyze its characteristics of simultaneous removal of nitrogen and carbon. The results showed that the optimal carbon source was sodium citrate; the optimal pH was 7.0; the optimal C/N was 17. In the reaction system of NH4Cl, KNO3 and NaNO2 for the only nitrogen source, Strain y6had better denitrification effect. The removal efficiencies were 99.67%, 100% and 99.20%. Strain y6could efficiently remove organic matter simultaneously in the process of denitrification, with COD removal rate of 82.17%, 95.75% and 97.83%, respectively. Almost no nitrate nitrogen and nitrite nitrogen accumulation in the y6 heterotrophic nitrification process. In the different mixing ratio of reaction system, the first were aerobic denitrification of nitrate nitrogen, Subsequent were ammonia nitrogen, nitrite nitrogen and COD removal. Ammonia nitrogen removal rate was only slightly lower in the presence of the nitrate nitrogen. The nitrite nitrogen affected y6heterotrophic nitrification process. The heterotrophic nitrification of y6had no effect o


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